Assay Methods for Clostridium perfringens Type A Enterotoxin
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چکیده
منابع مشابه
Assay methods for Clostridium perfringens type A enterotoxin.
Enterotoxin produced by a sporulating culture of Clostridium perfringens type A NCTC 8798 was purified to a level of 3,500 mouse mean lethal doses per mg of nitrogen. High-titer sera were obtained from rabbits injected with enterotoxin and used to compare the sensitivity of serological tests and bioassays for C. perfringens enterotoxin. Reversed passive hemagglutination was by far the most sens...
متن کاملUse of dogs as an assay for Clostridium perfringens enterotoxin.
Three techniques for using the dog as an assay organism for Clostridium perfringens enterotoxin are described. These are believed to be more convenient than ligated ileal-loop procedures.
متن کاملVero cell assay for rapid detection of Clostridium perfringens enterotoxin.
A rapid assay which measured the biological activity of Clostridium perfringens enterotoxin was developed. The method involved the rapid killing of Vero cells by enterotoxin produced by C. perfringens grown in Duncan and Strong sporulation medium. Serial dilutions of toxin were added to Vero cells either in suspension or grown as monolayers in wells of a 96-well cell tissue culture cluster plat...
متن کاملPurification and biochemical properties of Clostridium perfringens type A enterotoxin.
The sporulation-specific enterotoxin of Clostridium perfringens type A, which is the toxin active in human food poisoning, has been purified from extracts of sporulating cells. Highly purified enterotoxin was obtained by treatment of crude cell extract with ribonuclease for 30 min, followed by sequential chromatography on Sephadex G-100, Cellex T cellulose, and hydroxylapatite. Recovery was 65 ...
متن کاملAntibody against Clostridium perfringens type A enterotoxin in human sera.
Antibody against Clostridium perfringens type A enterotoxin was found in 82% of Brazilian and 65% of American serum samples.
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1973
ISSN: 0003-6919
DOI: 10.1128/aem.26.1.111-115.1973